Analyzing Lipidomic Profiling and Perceived Stress Data Collected in the Strong Heart Family Study

• Activity Number: 444 - SPEED: Statistics in Epidemiology Part 2
• Type: Contributed
• Date/Time: Wednesday, August 10, 2022 : 11:35 AM to 12:20 PM
• Sponsor: Section on Statistics in Epidemiology
• Author(s): Megan Eisele* and Guanhong Miao and Oliver Fiehn and Tauqeer Ali and Shelley A Cole and Amanda Fretts and Jason G Umans and Jessica Reese and Kimberly Malloy and Richard B Devereux and Lyle G Best and Barbara V Howard and Elisa T Lee and Jinying Zhao and Ying Zhang
• Companies: The University of Oklahoma Health Sciences Center and University of Florida, Gainesville and University of California, Davis and University of Oklahoma Health Sciences Center and Texas Biomedical Research Institute and University of Washington, Seattle and Georgetown University Medical Center and University of Oklahoma Health Sciences Center and University of Oklahoma Health Sciences Center and Weill Cornell Medicine and Missouri Breaks Industries Research Inc. and MedStar Research Institute and University of Oklahoma Health Sciences Center and University of Florida, Gainesville and University of Oklahoma Health Sciences Center
• Keywords: lipidomics; perceived stress; acylcarnitines; American Indians; Strong Heart Study

Abstract

Psychosocial stressors are cited as fundamental agents of worsened health and reduced quality of life. Previous studies report psychological stress produced changes in lipid concentrations, but the full spectrum of the lipidome related to perceived stress remains unexplored. Using the lipidome data from American Indians (n=1959) participating in the Strong Heart Family Study, this project aims to identify statistical methods for finding individual lipid elements associated with perceived stress. Untargeted LC-MS detected 1542 total lipids, 1024 unknown and 518 known lipids from 16 lipid classes. A Spearman’s rank correlation coefficient was calculated to assess the relationship between each measure of normalized, known lipid (n=518) and Cohen's Perceived Stress Scale (1983). False discovery rate was controlled to 0.05 using a Benjamini-Hochberg procedure. Multivariable linear mixed regression accounting for familial correlation was used for analysis where perceived stress was the independent variable and lipid level was the outcome. After adjusting for covariates, we identified 3 acylcarnitines, AC(10:1), AC(11:1), and AC(8:1), inversely associated with PSS (p< 0.05).