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Activity Number: 126 - SPEED: New Methods in Statistical Genomics and Genetics Part 1
Type: Contributed
Date/Time: Monday, July 29, 2019 : 8:30 AM to 10:20 AM
Sponsor: Section on Statistics in Genomics and Genetics
Abstract #307111 Presentation
Title: The Rabl Configuration Limits Topological Entanglement of Chromosomes in Budding Yeast
Author(s): Maxime Pouokam*
Companies: UC Davis Statistics Club
Keywords: Chromatin conformation; Genome architecture; Ralb configuration; Topology invariant; Linking proportion

The three dimensional organization of genomes remains mostly unknown due to their high degree of condensation. Biophysical studies predict that condensation promotes the topological entanglement of chromatin fibers and the inhibition of function. How organisms balance between functionally active genomes and a high degree of condensation remains to be determined. Here we hypothesize that the Rabl configuration, characterized by the attachment of centromeres and telomeres to the nuclear envelope, helps to reduce the topological entanglement of chromosomes. To test this hypothesis we developed a novel method to quantify chromosome entanglement complexity in 3D reconstructions obtained from Chromosome Conformation Capture(CCC) data. Applying this method to published data of the yeast genome, we show that computational models implementing the attachment of telomeres or centromeres alone are not sufficient to obtain the reduced entanglement complexity observed in 3D reconstructions. It is only when the centromeres and telomeres are attached to the nuclear envelope that the complexity of entanglement of the genome is comparable to that of the 3D reconstructions.

Authors who are presenting talks have a * after their name.

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