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Abstract:
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Motor proteins move cargo, such as vesicles and organelles, inside cells along a microtubule. Studying this intracellular transport can provide important insight into neurodegenerative diseases where this transport breaks down. One method for observing motor-cargo complexes is using gold-nanoparticles and dark field microscopy attached to the cargo, which allows for a sampling time on the order of one millisecond. Since microtubules are cylinders that we may only view from the side, the curvature can affect the perceived fluctuations of the particle position, posing challenges for inference. For such gold-nanoparticle data, we develop methods to predict movement of both motors and cargo when only the cargo is observed and develop associated inference techniques in order to uncover the underlying mechanisms of this cellular transport process.
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