Online Program Home
My Program

Abstract Details

Activity Number: 302 - Omics I
Type: Contributed
Date/Time: Tuesday, July 31, 2018 : 8:30 AM to 10:20 AM
Sponsor: Biometrics Section
Abstract #330605
Title: Modeling Dynamics of V(D)J Recombination in T Cell Formation
Author(s): Kingshuk Roy Choudhury*
Companies: Duke University, Dept. of Biostatistics and Bioinformatics
Keywords: VDJ recombination; high througput sequencing; T cell repertoire; constrained Brownian motion; numerical optimization; statistical modelling

Our body produces a large variety or 'repertoire' (over 100000 variants) of T cells, enabling our immune system to recognize a wide variety of antigens and thus organize a response against them. V(D) J recombination is the mechanism by which this large variety of T cells are produced from a relatively small section of the genome. This section contains about 80 contiguous V D segments followed by about 50 contiguous J segments (exact numbers depend on the species). During T cell maturation, this section of the genome is edited to produce a combination of a particular V and J segments to create a particular 'signature' capable of recognizing a specific type of antigen. The mechanism of editing isn't well understood. To discover this process, we model data obtained from a novel screen of a T cell repertoire in wild type and mutant mice. The screen utilizes high throughput sequencing of T cell genomes to obtain the joint distribution of V-J combinations in the repertoire at a given time. We propose a constrained Brownian motion with linear drift model for the dynamics of V segment usage relative to J locus. The model is fit by numerical optimization and fits the mean and variance of th

Authors who are presenting talks have a * after their name.

Back to the full JSM 2018 program