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Activity Number: 28 - SPEED: A Mixture of Topics in Health, Computing, and Imaging
Type: Contributed
Date/Time: Sunday, July 29, 2018 : 2:00 PM to 3:50 PM
Sponsor: Section on Statistics in Imaging
Abstract #329459 Presentation
Title: Remote Perconditioning Enhances Neuro Protection and Collateral Blood Flow During Ischemia in Distal Cerebral Ischemic Rat Model (MCAo) Through AMPK-ENOS Pathways
Author(s): Abdul Salam* and Aijaz Parray and yonglie Ma and Naveed Akhter and Sajitha VP and Ruth Priyanka and Ian Winship and Nosheen Shahid and Ashfaq Shuaib
Companies: Hamad Medical Corporation and Hamad Medical Corporation and University of Alberta and Hamamd Medical Corporation and Hamad Medical Corporation and Hamamd Medical Corporation and University of Alberta and Hamamd Medical Corporation and University of Alberta
Keywords: Middle Cerebral Artery occlusion; Remote Ischemic preconditioning; Laser Speckle Contrast Imaging; Activated Metabolism Sensing Protein Kinase; Western Blotting; Mann Whitney U-test
Abstract:

The mechanisms for protection behind Remote Ischemic Perconditioning (RIPerC) are not fully understood yet. In this study, we have investigated the neuroprotective efficacy and the role of AMPK- eNOS pathways in limb RIPerC using a distal Middle Cerebral Artery occlusion (MCAo) rat model. Adult male rats with and without MCAo followed by perconditioning were included. Laser Speckle Contrast Imaging (LSCI), Western Blotting (WB), RT-PCR techniques were used to measure collateral flow augmentation, pAMPK, and eNOS expression respectively. Mann Whitney U-test was used to compare the pAMPK and eNOS between control and RIPerC treated rats. LSCI revealed that RIPerC Augmented collateral flow into distal segments compared to control rats. Our results also showed that pAMPK increased significantly (p< 0.01) in RIPerC treated rats (89.99±2.05) compared to controls (34.24±1.05). eNOS was also significantly (p< 0.01) increased in RIPerC treated rats (2.04±0.13) compared to controls (0.87±0.08). Our results suggest that RIPerC Augmented collateral flow into distal segments could be neuroprotective and may be potentially due to the activation of pAMPK- eNOS pathways.


Authors who are presenting talks have a * after their name.

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