Online Program Home
My Program

Abstract Details

Activity Number: 467 - Statistical Advances for Cancer Genomics and Immunogenomics - from Single-Cell to Correlated Population
Type: Topic Contributed
Date/Time: Wednesday, August 1, 2018 : 8:30 AM to 10:20 AM
Sponsor: Biometrics Section
Abstract #328817 Presentation
Title: ScImpute: Accurate and Robust Imputation for Single Cell RNA-Seq Data
Author(s): Jingyi Li* and Wei Li
Companies: University of California, Los Angeles and University of California, Los Angeles
Keywords: Single cell RNA sequencing; Imputation; Mixture model; Nonnegative regression; Clustering; Differential expression analysis

The emerging single-cell RNA sequencing (scRNA-seq) technologies enable the investigation of transcriptomic landscapes at single-cell resolution. ScRNA-seq data analysis is complicated by excess zero counts, the so-called dropouts due to low amounts of mRNA sequenced within individual cells. We introduce scImpute, a statistical method to accurately and robustly impute the dropouts in scRNA-seq data. ScImpute automatically identifies likely dropouts, and only perform imputation on these values without introducing new bias to the rest data. ScImpute also detects outlier cells and excludes them from imputation. Evaluation based on both simulated and real human and mouse scRNA-seq data suggests that scImpute is an effective tool to recover transcriptome dynamics masked by dropouts. ScImpute is shown to identify likely dropouts, enhance the clustering of cell subpopulations, improve the accuracy of differential expression analysis, and aid the study of gene expression dynamics.

Authors who are presenting talks have a * after their name.

Back to the full JSM 2018 program