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Activity Number: 418 - Contributed Poster Presentations: Section on Statistics in Genomics and Genetics
Type: Contributed
Date/Time: Tuesday, August 1, 2017 : 2:00 PM to 3:50 PM
Sponsor: Section on Statistics in Genomics and Genetics
Abstract #324194
Title: Rates of DNA Mutation in Genes and Inte-Gene Regions
Author(s): Brian French* and Laszlo Csonka and Mark Ward and Nicole Markley
Companies: Purdue University and Purdue University and Purdue University and Purdue University
Keywords: Bioinformatics ; CLUSTALw ; Genetics
Abstract:

The aim of our analysis was to compare the rates of evolution of DNA sequences of genes and intergenic regions. The chromosomes of the related genera of bacteria, Escherichia coli K-12, Salmonella enterica serovar Typhimurium, and Citrobacter koseri, contain extensive regions in which the order of genes of identical. The fact that the gene order is so conserved suggests that both the genes and intergenic spacers were derived by evolution from corresponding sequences in the last common ancestor of the three organisms, and it enabled us to compare the sequence changes in functional genes and in intergenic spacers across the three organisms.

Pairwise comparisons using BLASTN were used to identify regions that had shared genes. ClustalW was then used to identify single base mutations in both genes and inter-gene regions. Mutation frequencies were determined for all paired comparisons. This analysis indicated that the mutation frequencies are significantly higher in intergenic spacers do not have specific sequence-dependent functions, and therefore, they can accumulate mutations more liberally than genes, which are constrained by their function.


Authors who are presenting talks have a * after their name.

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