Online Program Home
My Program

Abstract Details

Activity Number: 263
Type: Contributed
Date/Time: Monday, August 1, 2016 : 2:00 PM to 3:50 PM
Sponsor: Biopharmaceutical Section
Abstract #319826
Title: Design of Experiments for Hydrophobic Interaction Chromatography Optimization
Author(s): Na Zhang* and Lily Squibb Tsang and Kedar Dave and Joseph Calzada and Gregory A. Barker and Angela Lewandowski and Zhengjian Li
Companies: Bristol-Myers Squibb and Bristol-Myers Squibb and Bristol-Myers Squibb and Bristol-Myers Squibb and Bristol-Myers Squibb and Bristol-Myers Squibb and Bristol-Myers Squibb
Keywords: Hydrophobic interaction chromatography ; Design of Experiment ; Definitive screening design
Abstract:

Hydrophobic interaction chromatography (HIC) is a useful separation technique employed in manufacturing processes for protein therapeutics. HIC separates proteins from one another based on their properties of hydrophobicity, thus purifying proteins while maintaining the biological activity under certain conditions. Our interest is to study protein behavior in HIC and get the optimal conditions of HIC. Design of Experiment (DOE) is the most efficient way to solve this optimization problem during process development. An additional goal of this study is to compare multiple different types of DOEs including two Resolution III fractional factorial designs, two Resolution IV fractional factorial designs, Plackett-Burman design and definitive screening design. The comparison has two aspects. First, we illustrate the basic statistical properties of each design type. Second, the comparison is based on the optimization results of this real HIC example.


Authors who are presenting talks have a * after their name.

Back to the full JSM 2016 program

 
 
Copyright © American Statistical Association