Online Program Home
My Program

Abstract Details

Activity Number: 38
Type: Contributed
Date/Time: Sunday, July 31, 2016 : 2:00 PM to 3:50 PM
Sponsor: Biopharmaceutical Section
Abstract #319417
Title: Bioassay Case Study Applying the Maximin D-Optimal Design Algorithm to the Four-Parameter Logistic Model
Author(s): Todd Coffey*
Companies: Washington State University
Keywords: experimental design ; sigmoidal curve ; cytotoxicity ; nonlinear model ; optimality
Abstract:

Cell-based potency assays play an important role in the characterization of biopharmaceuticals but they can be challenging to develop in part because of greater inherent variability than other analytical methods. Our objective is to select concentrations on a dose-response curve that will enhance assay robustness. We apply the maximin D-optimal design concept to the four-parameter logistic (4PL) model and then derive and compute the maximin D-optimal design for a challenging bioassay using curves representative of assay variation. The selected concentration points from this 'best worst case' design adequately fit a variety of 4PL shapes and demonstrate improved robustness.


Authors who are presenting talks have a * after their name.

Back to the full JSM 2016 program

 
 
Copyright © American Statistical Association