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Abstract Details
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Activity Number:
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641
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Type:
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Topic Contributed
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Date/Time:
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Thursday, August 2, 2012 : 10:30 AM to 12:20 PM
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Sponsor:
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Biometrics Section
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| Abstract - #305510 |
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Title:
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Modeling Calibration of Targeted Mass Spectrometry Protein Measurements in Complex Fluids
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Author(s):
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Steven Skates*+
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Companies:
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MGH
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Address:
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Suite 560, Boston, MA, 02114-2517, United States
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Keywords:
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Targeted MS ;
Assay calibration ;
LOD ;
Assay CV
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Abstract:
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Targeted mass spectrometry (MS) approaches are a more rapid method for developing an assay for a protein in a complex fluid such as plasma or serum than traditional ELISAs. Calibration of the assay, assessing its accuracy, coefficient of variation, lower limit of detection, and other quantitative metrics, are important measures for determining the quality of the assay. Choices of target proteotypic peptide, background matrix for standards, and number of replicates potentially affect all the assay's metrics. We develop statistical models for assay calibration and provide example results from targeted MS approaches for plasma assays including multiple reaction monitoring (MRM) and Stable Isotope Standard Capture with Anti-Peptide Antibodies (SISCAPA).
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The address information is for the authors that have a + after their name.
Authors who are presenting talks have a * after their name.
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