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Abstract Details
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Activity Number:
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343
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Type:
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Contributed
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Date/Time:
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Tuesday, July 31, 2012 : 10:30 AM to 12:20 PM
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Sponsor:
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Biopharmaceutical Section
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| Abstract - #304746 |
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Title:
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A Statistical Approach to Determining the Minimum Bead Number for Luminex Bead--Based Assays
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Author(s):
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Yuan (Sally) Zhang*+ and Xiaoyu Jiang and Shuguang Huang
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Companies:
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Boehringer Ingelheim Pharmaceuticals, Inc. and Novartis Institutes for BioMedical Research and Precision Therapeutics, Inc.
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Address:
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900 Ridgebury Road, Ridgefield, CT, 06877, United States
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Keywords:
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microbead ;
minimum bead number ;
assay performance ;
Luminex
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Abstract:
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While recent advances in Luminex microbead technology have significantly improved bioassays' throughput, as a relatively new technology, one problem is the determination of the minimum bead number needed per analyte for reliable quantification. Current guidelines on this issue are mostly subjective and are without statistical foundation. In this paper, we use a statistical approach to investigate the relationship between bead number and assay performance that includes precision, bias and sensitivity. Our results suggest that the required number of beads is a function of multiple factors and depends on the analysis goals. This method can potentially provide a guide on bead number determination in assay development and also facilitate quality control for future experimental data.
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Authors who are presenting talks have a * after their name.
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