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Abstract Details
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Activity Number:
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425
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Type:
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Contributed
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Date/Time:
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Tuesday, August 2, 2011 : 2:00 PM to 3:50 PM
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Sponsor:
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Section on Risk Analysis
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| Abstract - #303299 |
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Title:
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Concentration-Response Estimation for High-Throughput Screening Data
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Author(s):
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Rhyne Woodrow Setzer*+
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Companies:
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Environmental Protection Agency
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Address:
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National Center for Computational Toxicology, ORD, Research Triangle Park, NC, ,
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Keywords:
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High-throughput ;
ToxCastT ;
concentration-response ;
P-splines ;
micro-titer ;
Bayesian
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Abstract:
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Toxicologists and risk assessors are exploring the use in environmental risk assessment of data from high-throughput screens in which multiple (hundreds or thousands) chemicals are tested in 96-well or larger micro-titer plates for activity against multiple (dozens to hundreds) assays in concentration-response designs. Assays are conducted using laboratory robots, and chemicals and concentrations are applied in systematic patterns on plates. The use of automation can lead to both systematic plate effects and variability best modeled as autocorrelated errors. This presentation discusses evaluation of systematic plate effects and estimation of concentration-response curves in high-throughput data taking into account both systematic and auto correlated errors, illustrated using assays from the ToxCastT project of the USEPA. P-splines estimated using Bayesian methods are used to adjust out plate effects and estimate concentration-response curves, and to estimate concentrations associated with specified changes from background for each chemicalXassay combination. Uncertainties are characterized with posterior intervals. This abstract does not necessarily reflect U.S. EPA policy.
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Authors who are presenting talks have a * after their name.
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