|
Abstract:
|
Neighboring genes, although not always involved in the same pathways, are still similarly regulated at the level of transcription via various histone modifications. We seek to develop a genome wide map of these higher-order (i.e. multi-gene) domains through a novel analysis of ChIP-seq histone modification data in mouse neural progenitor cells (NPCs). As a starting point, we have described the HOXa gene cluster with five modifications. To determine domains we characterized these five sets of ChIP-seq data using hierarchical clustering and heat-map visualization. With HMM algorithms, we were then able to determine the most probable domain status of each gene. We found evidence of multi-gene domains, characterized by similar patterns in these five modifications. As we expand our study, we will provide insight into the structure, organization and regulation of the mouse genome.
|
