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Abstract:
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MicroRNAs (miRNA) are small single stranded RNA molecules, found in eukaryotes, responsible for the regulation of gene expression at the level of translation. Herein we describe analytical steps undertaken for processing Luminex MicroRNA (miRNA) data. This platform uses a bead-based technology where a random number of beads per miRNA are used to assess miRNA abundance. Background correction and normalization were found to be important procedures needed to remove technical variation from the data. Further, replicate hybridizations are an important means for assessing quality. Similar to other technologies, significance testing can be performed using standard hypothesis testing procedures with resulting p-values used for estimating the false discovery rates (FDR). Results comparing normal, cirrhotic, and hepatocellular carcinoma tissues will be presented.
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