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Abstract:
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Currently there are two ways to assess beta-cell glucose-sensitivity with regard to insulin secretion: the commonly accepted hyperglycemic clamp technique and the simpler oral glucose or meal challenge test. Yet the first is expensive with high requirements of clinical centers, and the second has substantial variability. A graded glucose infusion (GGI) methodology comes as a compromise. Since beta-cells secret insulin and C-peptide with ratio 1, insulin secretion rate (ISR) can be estimated by deconvoluting C-peptide concentration using a two-compartmental model. To stabilize and avoid negative estimates of ISR, a regularized deconvolution method can be employed. The talk illustrates applying it in a real-world GGI study. The linear relationship was established between ISR and glucose concentration at various infusion rates, and the slope was used to assess beta-cell glucose sensitivity.
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