|
Activity Number:
|
242
|
|
Type:
|
Contributed
|
|
Date/Time:
|
Tuesday, August 5, 2008 : 8:30 AM to 10:20 AM
|
|
Sponsor:
|
ENAR
|
| Abstract - #300588 |
|
Title:
|
Comparisons of Titer Estimation Methods for Multiplexed Pneumococcal Opsonophagocytic Killing Assay
|
|
Author(s):
|
Deli Wang*+
|
|
Companies:
|
The University of Alabama at Birmingham
|
|
Address:
|
, , ,
|
|
Keywords:
|
Multiplexed opsonization assay ; Four parameters logistic model ; Titer estimation
|
|
Abstract:
|
Titer estimation is one of the major components of immunoassay and vaccine development. A multiplexed In vitro opsonization assay (MOPA) is widely accepted to quantitate Streptococcus pneumococcal antibodies to serotype-specific pneumococcal capsular polysaccharide. Titer estimation of OPA is one important component of standardization of OPA and the selected statistical method is a factor influencing accuracy and precision of titer estimation. We evaluated five titer estimation methods for pneumococcal OPA in terms of precision and accuracy using three data sets generated by specifically designed experiments. We concluded that the traditional direct method did not perform as well as the other four methods in terms of precision and accuracy of titer estimation. The 4PL method is an alternative choice for OPA titer estimation. UAB opsotiter was developed to implement OPA titer estimation.
|
- The address information is for the authors that have a + after their name.
- Authors who are presenting talks have a * after their name.
Back to the full JSM 2008 program |
