Array Analysis To Assess Effects of RNAi-Based Silencing in Relation to Flavonoid Transport

• Activity Number: 154
• Type: Contributed
• Date/Time: Monday, July 30, 2007 : 10:30 AM to 12:20 PM
• Sponsor: Biometrics Section
• Author(s): Robert Norton*+ and Mousumi Rath and Claudia Uhde-Stone
• Companies: California State University, East Bay and California State University, East Bay and California State University, East Bay
• Keywords: gene expression ; array analysis ; RNA ; lupin

Abstract

In many soils, low availability of phosphorus (P) is a limiting factor in crop cultivation. White Lupin (Lupinus albus L.) is a legume that has unique adaptations for extracting P from soil when other plants cannot. In response to P deficiency, white lupin forms densely clustered roots, referred to as proteoid (or cluster) roots. A previous cDNA array approach identified genes up-regulated in proteoid roots under P deficiency, including LaMATE (Lupinus albus multi drug and toxin efflux). The encoded protein displays homology to the MATE protein family of transporters. LaMATE has been shown to be also induced under other nutrient stresses. Furthermore, RNAi-based gene silencing was shown to effectively reduce LaMATE expression in transformed white lupin roots. Here, we are using nylon filter arrays to analyze gene expression in transgenic lupin roots silenced for LaMATE expression.