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Activity Number:
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154
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Type:
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Contributed
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Date/Time:
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Monday, July 30, 2007 : 10:30 AM to 12:20 PM
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Sponsor:
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Biometrics Section
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| Abstract - #309171 |
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Title:
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Sample-Size Determination for a Flow Cytometry Assay
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Author(s):
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Elaine A. McVey*+ and Perry Haaland and Friedrich Hahn
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Companies:
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BD Technologies and BD Technologies and BD Technologies
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Address:
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21 Davis Drive, Research Triangle Park, NC, ,
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Keywords:
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flow cytometry ; resampling ; statistical computing ; bioinformatics ; sample size ; detection limit
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Abstract:
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Flow cytometry is an important assay technology that has been limited by a lack of computational tools appropriate for use by statisticians and bioinformaticians. In this application, we demonstrate the use of a new open source software package to perform standard statistical analyses on flow cytometry data. In this case, cell numbers available for assay were limited, and sample size planning was required. Data was available from a calibration experiment which mimicked the expected experimental conditions and provided large numbers of observations. Using the flowCore package in R, these flow cytometry data were resampled to obtain proper statistical estimates of assay sensitivity parameters for small sample sizes.
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