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Activity Number:
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154
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Type:
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Contributed
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Date/Time:
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Monday, July 30, 2007 : 10:30 AM to 12:20 PM
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Sponsor:
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Biopharmaceutical Section
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| Abstract - #308754 |
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Title:
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Statistical Applications in High-Throughput siRNA Synthetic Lethality Screens
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Author(s):
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Yue Li*+ and Gopinath Ganji and Holly Yin and Quick Que and Kok Long Ang and Libin Ma and Song Wu and Greg Tucker-Kellogg
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Companies:
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Lilly Singapore Center for Drug Discovery and Lilly Singapore Center for Drug Discovery and Translational Genomics Research Institute and Translational Genomics Research Institute and Lilly Singapore Center for Drug Discovery and Lilly Singapore Center for Drug Discovery and Lilly Singapore Center for Drug Discovery and Lilly Singapore Center for Drug Discovery
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Address:
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1 Science Park Road 0401, Singapore, 117528, Singapore
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Keywords:
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RNAi ; HTS ; Z prime factor ; sigmoidal dose response model ; IC50
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Abstract:
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RNA interference (RNAi) by small interfering RNA (siRNA) and short hairpin RNA (shRNA) reagents has emerged as a powerful technique to knock down the expression of specific genes. This has accelerated target validation efforts in drug discovery and development, particularly through cell-based phenotypic screening assays. An example of such an application involves "synthetic lethality" experiments in combination with small molecules for target or biomarker discovery. We have used this approach in a genome-wide high throughput screen (HTS) and examples of various statistical considerations and applications throughout the HTS, including experimental design, quality control and hit selection, will be presented and discussed.
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