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Activity Number:
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25
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Type:
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Contributed
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Date/Time:
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Sunday, July 29, 2007 : 2:00 PM to 3:50 PM
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Sponsor:
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Biopharmaceutical Section
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| Abstract - #310159 |
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Title:
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Optimization of Dilution Scheme
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Author(s):
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Andrew Chen*+ and Harry Yang and Iksung Cho
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Companies:
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MedImmune, Inc. and MedImmune, Inc. and MedImmune, Inc.
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Address:
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One Medimmune Way, Gaithersburg, MD, 20878,
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Keywords:
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ELISA assay ; Serial dilution
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Abstract:
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The ELISA is a fundamental tool of clinical immunology, and is used both as screening test and potency assay. Based on the principle of antibody-antibody interaction, this test allows for easy visualization of results and can be completed without the additional concern of radioactive materials use. However, it is also well known that the ELISA assay is, in general, very variable. There are many sources of variations such as day, analytical, reagent and etc. that may contribute to the total variability. In this paper, through statistical modeling, we develop a method that allows us to choose an optimal dilution scheme for sample processing so as to reduce the overall assay variability. The theoretical results were verified through a simulation study.
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- The address information is for the authors that have a + after their name.
- Authors who are presenting talks have a * after their name.
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