|
Activity Number:
|
428
|
|
Type:
|
Contributed
|
|
Date/Time:
|
Wednesday, August 9, 2006 : 10:30 AM to 12:20 PM
|
|
Sponsor:
|
Biometrics Section
|
| Abstract - #307341 |
|
Title:
|
A Soft Endpoint for HIV-1Ba-l Growth in Cervical, Rectal, and Tonsular In Vitro Assays
|
|
Author(s):
|
Sandra Senneke*+ and Nicola Richardson-Harman and James Cummins and Carol Lackman-Smith and Christina Bromley and Patricia Reichelderfer
|
|
Companies:
|
BioStat Solutions Inc. and BioStat Solutions Inc. and Southern Research Institute and Southern Research Institute and BioStat Solutions Inc. and National Institute of Child Health & Human Development
|
|
Address:
|
5 Ridgeside Court, Suite 202, Mount Airy, MD, 21771,
|
|
Keywords:
|
in-vitro ; end point ; time series ; HIV ; assay ; multi-center
|
|
Abstract:
|
Measurement of virus growth over time is currently achieved by visual inspection of a growth curve. We introduce a soft endpoint calculation for in vitro HIV-1Ba-l growth in a time series. Virus replication was assessed using the HIV gag p24 protein assay. The objective of the soft endpoint is to determine the start of the phase of virus growth where maximum, and in some cases exponential growth, has been achieved. This was calculated as the last day when the change in p24 between two consecutive days was greater than the p24 SD for the entire assay. Calculations were based on data from a multi-center study, where virus growth was measured at approximately three day intervals for up to 15 days. The soft endpoint was then used in a binary growth categorization for each assay and candidate assay factors were tested using logistic regression analyses.
|
