|
Activity Number:
|
173
|
|
Type:
|
Other
|
|
Date/Time:
|
Monday, August 7, 2006 : 2:00 PM to 3:50 PM
|
|
Sponsor:
|
The ASA
|
| Abstract - #307659 |
|
Title:
|
Multidimensional Scaling Analysis To Study Temporal Transcriptome Fingerprint Clustering in Human Dendritic Cells Infected with Wild-Type and Chimeric Viruses
|
|
Author(s):
|
Yongchao Ge*+ and Ana Fernandez-Sesma and Thomas M. Moran and Stuart C. Sealfon
|
|
Companies:
|
Mount Sinai School of Medicine and Mount Sinai School of Medicine and Mount Sinai School of Medicine and Mount Sinai School of Medicine
|
|
Address:
|
Department of Neurology and Microbiology and Center for Translational Systems Biology, New York, NY, 10029,
|
|
Keywords:
|
multidimensional scaling ; clustering ; QPCR ; dentritic cells ; viruses
|
|
Abstract:
|
Our lab has measured temporal transcriptome fingerprints across many genes from different buffy coats of human dendritic cells (DC) from different donors infected with wild-type and chimeric viruses. Due to changes in interest in specific targets over time, many genes have missing values in qPCR measurements, which can be problematic for statistical analysis. To address the missing value problem, we construct a distance that only uses the common genes between the two buffy coats and that automatically normalizes qPCR measurements. We then apply multidimensional scaling analysis and find that the buffy coats are clearly clustered into three groups according to the type of virus infected. Thus, dimensionality reduction of complex transcriptional changes shows high correlation for the same DC exposures across different donors.
|
