Technical, Longitudinal, and Genetic Variation in Proteomic Analysis of Human Plasma

• Activity Number: 510
• Type: Contributed
• Date/Time: Thursday, August 10, 2006 : 8:30 AM to 10:20 AM
• Sponsor: Biopharmaceutical Section
• Author(s): Imola K. Fodor*+ and Todd H. Corzett and Megan Choi and Vicki L. Walsworth and Kenneth W. Turteltaub and Sandra L. McCutchen-Maloney
• Companies: Lawrence Livermore National Laboratory and Lawrence Livermore National Laboratory and Lawrence Livermore National Laboratory and Lawrence Livermore National Laboratory and Lawrence Livermore National Laboratory and Lawrence Livermore National Laboratory
• Keywords: proteomics ; gel electrophoresis ; technical variation ; genetic variation ; biomarker ; plasma

Abstract

While human plasma is an ideal sample for the discovery of disease biomarkers, its complexity presents a number of challenges. To date, no results have been published on the technical variation of two-dimensional difference gel electrophoresis (2D DIGE), or on the longitudinal and genetic variation of human plasma. We address this gap by presenting: 1) a technical variation study where one human plasma sample was processed and analyzed on 12 replicate gels, and 2) a longitudinal and genetic variation study where plasma from 11 subjects collected at 3 time points were analyzed in triplicate on 100 gels. Estimates of the spot-wise protein variance (overall, as well as its components such as dye, subject, and random error effects) quantify the sources of variation in 2D DIGE, and thus lay the foundation of future disease biomarker detection studies with human plasma. UCRL-ABS-218476.