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Activity Number:
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510
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Type:
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Contributed
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Date/Time:
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Thursday, August 10, 2006 : 8:30 AM to 10:20 AM
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Sponsor:
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Biopharmaceutical Section
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| Abstract - #306296 |
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Title:
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Microarray, PCR, and Northern Blot: a Comparison
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Author(s):
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Yongzeng Ding and Borko Jovanovic*+ and Raymond Bergan and Irene Helenowski
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Companies:
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Northwestern University and Northwestern University and Northwestern University and Northwestern University
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Address:
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680 N. Lake Shore Drive, Chicago, IL, 60611,
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Keywords:
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prostate ; cancer ; reproducibility ; lab techniques ; gene expression
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Abstract:
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We provide a comparison of gene expression measurements (from native RNA) among Microarray (MA), quantitative polymerase chain reaction (PCR) and Northern blot (NB) measurements on two cell lines (PC3, PC3M) with two measurements each. Measurements are made on twenty-six "randomly selected" genes. In addition, we have two MA and PCR measurements of same genes from amplified RNA. Using NB as gold standard we describe and discuss variability and co-relation observed in these measurements.
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