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Abstract:
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Fluorescence-based microscopy technology has made it possible for cell-based, high-content imaging (HCI), which allows measurements of the intracellular fluorescence intensity of multiple targets on a cell-by-cell basis. In the context of drug discovery, HCI technologies are useful in profiling drug action in cellular milieu. Researches often are interested in whether a drug exerts a statistically significant effect, which is reflected by the perturbation of the distribution of certain phenotype. With the daunting amount of data, quantifying the treatment effect is a nontrivial challenge. This discussion will focus on background introduction of cell-based, high-content imaging and data analysis issues and statistical methodologies.
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