JSM 2005 - Toronto

Abstract #304212

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Legend: = Applied Session, = Theme Session, = Presenter
Activity Number: 184
Type: Topic Contributed
Date/Time: Monday, August 8, 2005 : 2:00 PM to 3:50 PM
Sponsor: Section on Statistics in Epidemiology
Abstract - #304212
Title: Microarray Gene Expression Using Resonance Light Scattering Nanoparticles
Author(s): Samir Lababidi*+ and Daya G. Ranamukhaarachchi
Companies: U.S. Food and Drug Administration and U.S. Food and Drug Administration
Address: Division of Biostatistics CDRH, Rockville, MD, 20850, United States
Keywords: resonance light scattering nanoparticles ; nanotechnology ; microarrays ; gene expression
Abstract:

Commonly used microarrays with signals generated using fluorescent molecules (e.g., Cy3 and Cy5) usually require either invasive sampling or RNA amplification to sufficiently produce accurate signals. In this talk, we present an alternative novel nucleic acid labeling method using nonfluorescent, nanometer-sized metal particles (Resonance Light Scattering [RLS] particles), generating high-intensity microarray gene expression signals. Using our FDA/CDRH microarray facility, microarray data generated using both direct fluorescent labeling and RLS nanoparticles were compared based on different starting total RNA and time required for hybridization with different printing surfaces. Here, we present the statistical experimental design used to appropriately estimate the unknown parameters necessary for extracting the gene signal. We will show that microarray gene expression using RLS nanoparticles has the potential to improve the detection of gene expression measurements, especially for low-abundance genes, and speed up the process for microarray data generation.

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Revised March 2005