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Activity Number:
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227
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Type:
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Contributed
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Date/Time:
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Tuesday, August 10, 2004 : 10:30 AM to 12:20 PM
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Sponsor:
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Section on Bayesian Statistical Science
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| Abstract - #302026 |
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Title:
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Gene Expression Patterns during Somatic Embryogenesis in Maize Tissue Culture: A Bayesian Approach
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Author(s):
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Tanzy M. Love*+ and Alicia Carriquiry and Ping Che and Stephen Howell and Bronwyn Frame and Kan Wang
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Companies:
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Iowa State University and Iowa State University and Iowa State University and Iowa State University and Iowa State University and Iowa State University
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Address:
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140 University Village Apt. E, Ames, IA, 50010,
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Keywords:
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gene expression ; maize ; somatic embryogenesis
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Abstract:
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Regeneration in tissue culture is important for improvement of maize using genetic engineering approaches. However, many important maize lines are difficult to regenerate. The program of gene expression during somatic embryogenesis in tissue culture was examined in regeneration-proficient lines in the effort to find key genes that may limit regeneration in more recalcitrant lines. Somatic embryos were generated from eight embryogenic callus lines developed from immature Hi II embryo explants using protocols developed and utilized at the Plant Transformation Facility at Iowa State.The callus lines were pooled into three groups, and RNA was extracted from samples at six time points during incubation under culture conditions that induce embryo formation, maturation, and germination. RNA was hybridized to 12K gene cDNA microarray chips produced by the Microarray Facility in the Center for Plant Genomics at Iowa State. Statistical design, background cleaning, and normalization issues are explored. Bayesian methods are implemented to detect significant gene expression patterns.
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