JSM 2004 - Toronto

Abstract #301718

This is the preliminary program for the 2004 Joint Statistical Meetings in Toronto, Canada. Currently included in this program is the "technical" program, schedule of invited, topic contributed, regular contributed and poster sessions; Continuing Education courses (August 7-10, 2004); and Committee and Business Meetings. This on-line program will be updated frequently to reflect the most current revisions.

To View the Program:
You may choose to view all activities of the program or just parts of it at any one time. All activities are arranged by date and time.

The views expressed here are those of the individual authors
and not necessarily those of the ASA or its board, officers, or staff.

Back to main JSM 2004 Program page
Activity Number: 194
Type: Contributed
Date/Time: Tuesday, August 10, 2004 : 8:30 AM to 10:20 AM
Sponsor: Biometrics Section
Abstract - #301718
Title: Methods for Evaluating T Cell Repertoire via CDR3 Spectratyping Analysis
Author(s): Susan M. Geyer*+ and Matthew Maurer
Companies: Mayo Clinic and Mayo Clinic
Address: 200 First St. SW, Rochester, MN, 55905,
Keywords: immunology ; spectratyping ; T cell repertoire
Abstract:

Analysis of T cell repertoires (TcR) has been the focus of recent studies of immunologic health. Diversity of this repertoire can effectively be measured through spectratyping, an RT-PCR-based method for estimating V gene representation and diversity of complementarity determining regions 3 (CDR3s). Primary focus has been on graphical analyses of chromatographs, where normal TcRs exhibit an array of variable CDR3 lengths for a single rearranged V gene. Chromatographs could be used for evaluating a few patients, but for larger studies is unfeasible. Thus, we developed methods to summarize key features of spectratyping data and methods for TcR analysis where three primary measures are used to quantify the TcR diversity and health. Our approach focuses on quantifying the total number of peaks representing individual CDR3 lengths, the proportion of peaks representing productively rearranged alpha and beta transcripts, and disturbances in the distribution of CDR3 lengths. Examples of these methods to identify patterns in T cell diversity across different age groups as well as between healthy subjects and cancer patients will be presented.

  • The address information is for the authors that have a + after their name.
  • Authors who are presenting talks have a * after their name.

Back to the full JSM 2004 program

JSM 2004 For information, contact jsm@amstat.org or phone (888) 231-3473. If you have questions about the Continuing Education program, please contact the Education Department.
Revised March 2004