Abstract #302308

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JSM 2003 Abstract #302308
Activity Number: 334
Type: Contributed
Date/Time: Wednesday, August 6, 2003 : 8:30 AM to 10:20 AM
Sponsor: Biometrics Section
Abstract - #302308
Title: Microarray Experiment Design and Replication Strategies
Author(s): Caimiao Wei*+ and James L. Rosenberger
Companies: Pennsylvania State University and Pennsylvania State University
Address: 12 Graduate Cir. Apt. A, State College, PA, 16801-5811,
Keywords:
Abstract:

Microarray experiments generate high-dimensional response output from typically a small number of experimental units, the microarray arrays. This presentation investigates the required number of biological replicates, the number of arrays per biological sample, and the number of replicate cDNA spots per array needed for an experiment on Arabidopsis (Fedoroff 2002) exposed to varying durations of ozone stress. The response is the expression level of specific genes. Two statistical problems are addressed. First, the multiple comparison issues arising from the very high dimensional response vector, in this case over 1,400 potential genes. Second, we address the choice of experimental design for efficient and cost-effective statistical confirmation of the potential regulatory genes. We show the sample size needed to significantly demonstrate an n-fold expressed gene for a given power. The design depends on the variance components among replicate biological samples, replicate arrays, and replicate cDNA spots on each array, and the relative costs of these replicates.

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