Abstract #302340

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JSM 2003 Abstract #302340
Activity Number: 307
Type: Invited
Date/Time: Wednesday, August 6, 2003 : 8:30 AM to 10:20 AM
Sponsor: IMS
Abstract - #302340
Title: Machine Vision Methods for DNA Microarray Spotting
Author(s): Javier F. Cabrera*+
Companies: Rutgers University-Hill Center
Address: Dept. of Stat., Piscataway, NJ, 08855,
Keywords:
Abstract:

DNA microarray row images are the result of scanning a DNA chip with a laser scanner in order to detect and measure the fluorescent signal at each pixel. The image contains an array of approximately circular spots representing a set of genes. Each of these circular spots is made of a few hundred pixels and the overall fluorescence signal of each group of pixels measures the abundance of the individual gene in the sample of a microarray experiment. We will discuss the following issues and propose methods to resolve them: 1) Spot detection by image segmentation and a comparison with other methods such as seed growing algorithms. 2) Array quality control. Background image analysis to check for patterns that would detect errors in the experiment. The methodology will be illustrated with several microarray examples.

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Revised March 2003