Abstract #301004

This is the preliminary program for the 2003 Joint Statistical Meetings in San Francisco, California. Currently included in this program is the "technical" program, schedule of invited, topic contributed, regular contributed and poster sessions; Continuing Education courses (August 2-5, 2003); and Committee and Business Meetings. This on-line program will be updated frequently to reflect the most current revisions.

To View the Program:
You may choose to view all activities of the program or just parts of it at any one time. All activities are arranged by date and time.

The views expressed here are those of the individual authors
and not necessarily those of the ASA or its board, officers, or staff.

Back to main JSM 2003 Program page
JSM 2003 Abstract #301004
Activity Number: 296
Type: Contributed
Date/Time: Tuesday, August 5, 2003 : 2:00 PM to 3:50 PM
Sponsor: Biopharmaceutical Section
Abstract - #301004
Title: Statistical Properties of In Vitro Assay Test-Retest Studies Used in Drug Discovery Research
Author(s): Brian J. Eastwood*+
Companies: Eli Lilly & Company
Address: Statistical and Mathematical Sciences, Indianapolis, IN, 46285-0001,
Keywords: agreement measures ; reliability ; test-retest studies ; in vitro assay
Abstract:

Test-retest studies have been proposed for examination of intra-assay variation in potency estimates derived from concentration-response assays (Eastwood et al. 2001, 2002). These studies supplement simpler studies designed to estimate the signal window (Sittampalam et al. 1997) and Z-factor (Zhang et al. 1998) criterion used in validation of high-throughput screening single-concentration assays. The standard protocol is to test 20-30 compounds in each of two runs of the assay to examine the assay variability. The analysis methods, adapted from Bland and Altman (1986), give three summary measures: the Limits of Agreement, Mean Ratio and Minimum Significant Ratio. These outcomes have been shown to identify unacceptable assays that otherwise would pass the single point signal window and Z-factor acceptance criterion (Eastwood 2002). In this presentation the statistical properties of the test-retest study outcomes are examined, and the proposed target criterion for each outcome is judged for suitability.

  • The address information is for the authors that have a + after their name.
  • Authors who are presenting talks have a * after their name.

Back to the full JSM 2003 program

JSM 2003 For information, contact meetings@amstat.org or phone (703) 684-1221. If you have questions about the Continuing Education program, please contact the Education Department.
Revised March 2003