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Activity Number:
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346
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Type:
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Topic Contributed
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Date/Time:
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Wednesday, August 14, 2002 : 2:00 PM to 3:50 PM
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Sponsor:
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Biopharmaceutical Section*
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| Abstract - #300682 |
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Title:
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Comparisons of Gene Expression Indexes for Oligonucleotide Arrays
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Author(s):
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Fred Wright*+ and William Lemon and Ralf Krahe and Jeff Palatini
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Affiliation(s):
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University of North Carolina, Chapel Hill and Ohio State University and U. T. M. D. Anderson Cancer Center and Ohio State University
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Address:
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3107B McGavran-Greenberg Hall, CB#7420, Chapel Hill, North Carolina, 27599, USA
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Keywords:
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gene expression ; microarray ; Affymetrix Genechip
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Abstract:
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Oligonucleotide expression arrays exhibit systematic and reproducible variation produced by the multiple distinct probes used to represent a gene. We present analytic results on the efficiency of model-based estimates of expression and develop a framework for empirical comparison of the estimates. Supporting data from a mixing experiment was generated using the Affymetrix platform. Statistical arguments using data from spiked control genes provides evidence that over 90% of the genes on the array are detectably transcribed, and that the model-based estimates can accurately detect the presence vs. absence of a gene. We discuss extensions to this work, including improved models and new study designs, and we present preliminary results on the effect of probe sequence content on hybridization intensity.
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