Abstract #300250

The views expressed here are those of the individual authors
and not necessarily those of the ASA or its board, officers, or staff.

Back to main JSM 2002 Program page
JSM 2002 Abstract #300250
Activity Number: 373
Type: Topic Contributed
Date/Time: Thursday, August 15, 2002 : 8:30 AM to 10:20 AM
Sponsor: Section on Statistical Computing*
Abstract - #300250
Title: Experimental Design Issues Affecting the Assessment of Differential Expression
Author(s): Christina Kendziorski*+
Affiliation(s): University of Wisconsin, Madison
Address: 1300 University Ave (6729 MSC), Madison, Wisconsin, 53703, USA
Keywords:
Abstract:

In an attempt to reduce subject-to-subject variability, mRNA samples are often pooled across subjects. Under some conditions, this can decrease measurement variability and in so doing reduce the number of replicates required. However, owing to array specific effects, pooling does not eliminate the need for replication. Furthermore, if contaminated samples are present, pooling can lead to unbiased estimates of expression. We will discuss conditions under which it is advantageous to pool mRNA samples and present experimental pooling designs, which provide for maximum measurement accuracy using a minimum number of arrays. The impact of each design on the assessment of differential expression will also be discussed.

  • The address information is for the authors that have a + after their name.
  • Authors who are presenting talks have a * after their name.

Back to the full JSM 2002 program

JSM 2002

For information, contact meetings@amstat.org or phone (703) 684-1221.

If you have questions about the Continuing Education program, please contact the Education Department.

Revised March 2002