Abstract:
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Raman inelastic scattering is associated with distinctive vibrational modes of a molecule. Measured Raman spectrum provides information about the bonds and molecular structures. Continuous efforts have been focused on developing novel data processing to extract accurate and relevant information from raw spectra. Here, we report results of intensity correlation analysis (ICA) of Raman spectra of Ficoll and Alginate samples prepared at different concentrations. While both Ficoll and Alginate are polysaccharide, their molecular structures are different -branched and linear. Their spectra display complex set of peaks attributed to the vibrational modes of Ficoll and Alginate molecules, water molecules, and oxygen and nitrogen molecules. For Ficoll solutions, ICA shows an overall linear trend, indicating similarity of the spectra. At high concentration (~1000 mg/ml) ICA shows a closed loop, due to shift of certain peaks. Comparison of Alginate and Ficoll spectra show significant differences in the 800-1500 cm-1 range but similar patterns at wavenumber large 1500 cm-1. The results demonstrate ICA as a potential insightful tool in chemometrics analysis of crowded biological samples.
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