Abstract Details
Activity Number:
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637
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Type:
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Topic Contributed
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Date/Time:
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Thursday, August 7, 2014 : 10:30 AM to 12:20 PM
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Sponsor:
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Section on Medical Devices and Diagnostics
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Abstract #311394
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View Presentation
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Title:
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Coefficient of Variation (Cv) - What Does it Measure in a Quantitative Polymerase Chain Reaction (Qpcr) Assay?
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Author(s):
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Jeng Mah*+
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Companies:
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Beckman Coulter
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Keywords:
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Coefficient of variation ;
Precision and reproducibility ;
Measurement scales ;
Clinical applicability
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Abstract:
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In a real-time qPCR assay, the measurand, typically a trace amount of a certain DNA or RNA molecule, is replicated repeatedly for measurement. The original concentration of the target molecule X0 is quantified by the number of cycles Ct taken to reach a predetermined threshold Xt = X0 [1 +E]Ct, where E is a replication efficiency constant. This mechanism suggests a logarithmic link between the observable random variables, Ct, and the clinically meaningful concentration (X0). Conventionally, log10(X0), i.e. log10(X0) = log10(Xt) - Ct log10(1+E), and X0 are both used in reporting qPCR assay results. While X0 and log10(X0) are easily interchangeable, their statistics-especially the coefficient of variation, CV, which is an important measurement for precision and reproducibility of an assay-are not, either in magnitude or in meaning.
In this study, we characterize algebraic and interpretational differences between CVs computed from X0 and log10(X0) for evaluating the reproducibility of a qPCR assay. Effectiveness of different approaches given the presence of random measuring errors is discussed.
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Authors who are presenting talks have a * after their name.
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