This is the program for the 2010 Joint Statistical Meetings in Vancouver, British Columbia.
Abstract Details
Activity Number:
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281
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Type:
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Topic Contributed
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Date/Time:
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Tuesday, August 3, 2010 : 8:30 AM to 10:20 AM
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Sponsor:
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Section on Statistical Learning and Data Mining
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Abstract - #307713 |
Title:
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Developing Targeted Protein Measurement for Label-Free Multiple Reaction Monitoring
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Author(s):
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Dean Billheimer*+
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Companies:
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The University of Arizona
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Address:
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1657 E Helen St., Tucson, AZ, 85721,
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Keywords:
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proteomics ;
multiple reaction monitoring ;
biomarker verification ;
label-free quantitation ;
analytical sensitivity ;
stable-isotope dilution
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Abstract:
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Liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM-MS) provides a powerful tool for targeted protein quantitation. The most common implementation of LC-MRM-MS uses stable isotope dilution (SID), in which synthetic, isotopically labeled peptides serve as internal standards for all target peptides. Implementation of the SID-based approach is limited by the cost of acquiring standards, particularly when application to biomarker verification may require configuration of dozens to hundreds of assays. I evaluate performance of SID together with alternative methods that do not require isotopically labeled version of target peptides. I develop a measure of technical merit for comparing measurement methods, and show its extension to the multivariate calibration setting for MRM.
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Authors who are presenting talks have a * after their name.
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