This is the program for the 2010 Joint Statistical Meetings in Vancouver, British Columbia.
Abstract Details
Activity Number:
|
157
|
Type:
|
Topic Contributed
|
Date/Time:
|
Monday, August 2, 2010 : 10:30 AM to 12:20 PM
|
Sponsor:
|
Biometrics Section
|
Abstract - #307482 |
Title:
|
Isoform Detection from RNA-seq with Restriction Enzyme Fragmentation
|
Author(s):
|
Naomi S. Altman*+ and Qingyu Wang and Aleksandra B. Slavkovic and Vishesh Karwa
|
Companies:
|
Penn State and Penn State and Penn State and Penn State
|
Address:
|
312 Thomas Bldg, University Park, PA, 16802-2111,
|
Keywords:
|
gene expression ;
RNA-seq ;
isoform ;
bioinformatics ;
differential expression
|
Abstract:
|
Genes in complex organisms may encode multiple mRNAs, called isoforms which result in different proteins. Isoform expression is often more relevant than gene expression. The advent of ultra-high throughput sequencing technology has made it possible to directly sequence large numbers of RNA fragments, obtaining a direct measure RNA abundance. Restriction enzyme fragmentation reliably produces a single sequenced fragment per RNA, providing a direct measure of gene expression from tag counts. However, since each restriction site can belong to multiple isoforms, the counts must be partitioned among the isoforms to derive accurate isoform counts. We combine a model for fragmentation with methodology for contingency table analysis to estimate isoform abundance taking into consideration differing probabilities of restriction failure for each isoform.
|
The address information is for the authors that have a + after their name.
Authors who are presenting talks have a * after their name.
Back to the full JSM 2010 program
|
2010 JSM Online Program Home
For information, contact jsm@amstat.org or phone (888) 231-3473.
If you have questions about the Continuing Education program, please contact the Education Department.