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Activity Number:
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538
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Type:
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Invited
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Date/Time:
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Thursday, August 6, 2009 : 8:30 AM to 10:20 AM
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Sponsor:
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Section on Physical and Engineering Sciences
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| Abstract - #303015 |
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Title:
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Understanding Heterogeneity of Chemical Localization in Live Cells
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Author(s):
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Kerby Shedden*+
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Companies:
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University of Michigan
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Address:
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Department of Statistics, Ann Arbor, MI, 48109-1107,
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Keywords:
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Cell imaging ; Image analysis ; High content screening ; Microscopy ; Biological heterogeneity ; Chemical biology
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Abstract:
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A major determinant of the effects of chemical probes when introduced to living systems is whether and where they localize in cells. Microscope images of live cells incubated with chemically diverse probes provide experimental data for this question, but analysis of these relatively low-contrast images is challenging. A striking phenomenon is that cells in an image from the same lineage, exposed to the same concentration of probe, often exhibit different localization behaviors. I will discuss our approach to characterizing this variation across cells in an image. We considered whether the variation in localization behavior can be explained in terms of differences in the cells that are apparent in the pre-treatment images. Another key question is whether the degree of heterogeneity across cells exposed to a particular probe is to some degree determined by the probe's chemical structure.
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- The address information is for the authors that have a + after their name.
- Authors who are presenting talks have a * after their name.
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