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Activity Number:
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285
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Type:
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Contributed
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Date/Time:
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Tuesday, July 31, 2007 : 10:30 AM to 12:20 PM
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Sponsor:
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Biopharmaceutical Section
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| Abstract - #310355 |
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Title:
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Proteomic Analysis by 2-D PAGE for Response to Low-Dose Ionizing Radiation and Arsenic Exposure
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Author(s):
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Dan Li*+ and Susanne R. Berglund and Alison Santana and Zelanna Goldberg and David Rocke
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Companies:
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University of California, Davis and University of California, Davis and University of California, Davis and University of California, Davis and University of California, Davis
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Address:
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6600 Orchard Park Circle, Apt 6024, Davis, CA, 95616,
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Keywords:
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Protein expression data ; 2-D PAGE ; Statistical differential analysis ; Empirical Bayes estimate ; Missing data ; EM algorithm
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Abstract:
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Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is an important technique in proteomics. We have developed a procedure to analyze protein expression data generated by 2-D PAGE to identify the proteins that are differentially expressed across different conditions. The procedure consists of data normalization, variance stabilization, analysis of variance, estimate of protein-specific variance, adjustment for multiple comparisons and a Chi-square test. Missing data is a common problem encountered by researchers when using 2-D PAGE. Each missing spot is either left-censored or just randomly missing. A maximum likelihood model is set up which accounts for both of these two causes of missing. A case study on the effect of low dose arsenic and low dose ionizing radiation exposure is presented.
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- The address information is for the authors that have a + after their name.
- Authors who are presenting talks have a * after their name.
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